Use of hr3 enhancer and P74 TM domain in baculovirus surface display

摘要

Baculovirus surface display technique provides a new platform for novel vaccine research and production. Unfortunately, the low display efficiency in current methods and the waste of occlusion-derived virion (ODV) products limited its application. We investigated the use of two motifs, BmNPV hr3 and transmembrane domain of P74 (P74TM), in display. Budding virus (BV) with hr3 showed a 14.2-time enhanced display efficiency than the current recombinant BV. Hemagglutinin (HA) protein of H5N1 influenza virus was displayed by using 3 different vectors to ensure the improvement of display efficiency and the characters of displayed protein in recombinant baculovirus. Immunoassay demonstrated that the recombinant BV with TM/CTD of vsvG protein, and hr3 could induce the highest level of neutralizing antibody against HA, suggesting that the optimized HA displaying BV could be a novel live virus-based vaccine candidate for influenza virus. In addition, GFP fused with the P74TM could be anchored to the ring zone in infected cell and the ODV envelope, which may luminate a new direction for ODV display and provide a promising strategy to use ODV products.