Modern proton-detected 1D H-1-N-15 NMR experiments. Application to the measurement of H-1,N-15 coupling constants at natural abundance

摘要

Non-selective and selective versions of several proton-detected 1D NMR experiments to be applied to N-15 are proposed. Clean, artifact-free 1D spectra are easily obtained by the effective coherence selection by pulsed-field gradients and the attainable sensitivity is maximized using modern pulse schemes. Despite the low sensitivity inherent to N-15 NMR spectroscopy, the successful application of these experiments is demonstrated for resonance assignments and accurate measurement of both one-bond and long-range proton-nitrogen coupling constants on a model tripeptide at natural abundance. Copyright (C) 2001 John Wiley Sons, Ltd. [References: 30]