Covalent Heparin Modification of a Polysulfone Flat Sheet Membrane for Selective Removal of Low-Density Lipoproteins: A Simple and Versatile Method

摘要

A simple, convenient and economical method for the heparinization of PSf membranes is described, with the aim of preparing an LDL adsorber for simultaneous LDL apheresis and hemodialysis. An atmospheric pressure glow discharge generator is used to activate the PSf membrane surface, with subsequent chemical binding of heparin in the presence of EDC and NHS. ATR-FTIR spectroscopy and XPS measurements confirm successful surface modification. The PSf-Hep membrane shows good blood compatibility, with a relatively low amount and normal morphology of adherent platelets. ELISA results indicate that the PSf-Hep membrane exhibits excellent selective affinity for LDL in single and binary protein solutions, suggesting potential applications in hemodialysis with simultaneous LDL removal. A simple and economical method is described for the heparinization of polysulfone membranes, aiming at preparing a low-density lipoprotein absorber for simultaneous LDL apheresis and hemodialysis. CA and TB colorimetric tests demonstrate improved hydrophilicity of the heparinized membranes, while EILSA tests show excellent LDL affinity.