Effect of Molecular Weights of Polyethyleneimine on the Polyplex Formation with Calf Thymus DNA

摘要

In this study we investigated the spectral properties,including electric absorption,circular and linear dichroism (CD and LD),and fluorescence emission,of DNA in a DNA-branched polyethyleneimine (BPEI) polyplex at various polymer molecular weights (M_w) and BPEI-amine-to-DNA-phosphate ratios (N/P ratios).All BPEIs exhibited a common N/P dependence in their absorption and CD spectra.At N/P ratios < 1.0,we observed some hyperchromism in the absorption spectrum,red-shifts in CD bands,and decreases in LD intensity and fluorescence intensity of intercalated ethidium.At intermediate N/P ratios,complete collapse of all spectra occurred.As the N/P ratio increased further,the polyplex dissolved in water.From its characteristic CD spectrum obtained under these conditions,we conclude that the DNA exists in a B-like form.The fluorescence and LD intensities never recovered-even at high N/P ratios- which indicates that the dissolved polyplex possesses positive charges and the DNA in the polyplex is condensed despite its B-form CD spectrum.The N/P range in which the absorption and CD signals collapsed was wider when the BPEIs M_w decreased.In the case where the BPEIs M_w.was 0.8 k,recovery of the absorption and CD spectral properties at a high N/P ratio was never achieved,which suggests that the molecular weight of the polymer plays an important role in its dissolution at a high N/P ratio.