A Rapid Quantification of beta-Carotene in Fruits and Vegetables by Dispersive Liquid-Liquid Microextraction Coupled with UV-Vis Spectrophotometry: Optimized by Response Surface Methodology

摘要

A simple, fast, and efficient method consisted of optimized dispersive liquid-liquid microextraction (DLLME) followed by UV-vis spectrophotometry was developed for determination of beta-carotene in fruits and vegetables. Chloroform and methanol were chosen as extraction and disperser solvents, respectively. The extraction process was optimized using a central composite design (CCD) with the optimum points of 115 mu L for volume of extraction solvent and 6.5 % (w/v) for salt concentration. Under the optimal conditions, the relative standard deviation (RSD, C = 500 mu g L-1, n = 5), limit of detection (LOD), linear dynamic range (LDR), and coefficient of determination (R-2) were 1.08 %, 2 mu g L-1, 50-1,500 mu g L-1, and 0.991, respectively. The present method consisted of a simple and fast sample preparation procedure without any antioxidant addition, saponification, and purification was used.