Comparison and validation of rutin and quercetin contents according to the extraction method of tartary Buckwheat (Fagopyrum tataricum Gaertn.)

摘要

The stability and accuracy of ultra-performance liquid chromatography (UPLC) used for evaluating the contents of rutin and quercetin in tartary buckwheat (Fagopyrum tataricum Gaertn.) seeds extracted by seven different extraction methods were determined. The seven extraction methods were reflux extraction (RE), ultra-sonification extraction (UE), stirrer extraction (SE), RE after UE (UE+RE), RE after SE (SE+RE), UE after SE (SE+UE), and RE with UE after SE (SE+UE+RE). Among the seven extraction methods used, RE yielded comparatively higher contents of rutin (2,277 mg/ 100 g) and quercetin (158mg/100g) than those yielded by other six extraction methods. The intra-day repeatability and inter-day precision of RE was 0.4-3.2 considering relative standard deviation (RSD), while accuracy was 88.8-102.4. Therefore, RE with UPLC would be a rapid, accurate, and stable method for analyzing rutin and quercetin contents in tartary buckwheat.