Behavior of histamine-producing bacteria in shimesaba, raw mackerel salted and marinated in vinegar during processing and storage at various temperatures.

摘要

To study the behavior of histamine-producing bacteria during the processing and storage of shimesaba, we inoculated histamine-producing bacteria into raw mackerel and then evaluated bacterial counts and histamine content at each shimesaba processing step. Six histamine-producing bacteria-Morganella morganii, Enterobacter aerogenes, Raoultella planticola, Photobacterium damselae (2 strains), and Photobacterium phosphoreum-were inoculated onto the surface of mackerel fillets at various cell densities (no inoculation, 104, and 107 cfu ml-1). The fillets were then treated according to the common procedure used for processing shimesaba. The concentration of NaCl in brine during the salting procedure was 10 (w/v), and the main ingredients of the marinade broth were 27 vinegar, 3.8 sucrose, 0.3 sodium glutamate, and 0.1 sodium succinate. The level of histamine-producing bacteria and of histamine did not increase during the salting and marinating procedures. However, during the storage tests, histamine content increased remarkably in the shimesaba products stored at 10 degrees C for 3 days, and organoleptical degradation was also observed. However, significant deterioration, based on bacterial count, histamine content, and organoleptic evaluation, was not observed in samples stored at 5 degrees C. These results indicate that the use of traditional marinade broth during the processing of shimesaba and storage below 5 degrees C are effective methods for preventing histamine accumulation