The Single Cell-to-Ct~(TM) Kit: A Simplified Method for Expression Analysis in Individual Cells The Current State of Single Cell Analysis

摘要

"No two cells are the same" is a saying that many scientists have used to explain the importance of single cell analysis. When looking at cell populations as a whole, the differences between cells are masked and averaged into a single profile. Even though there are many distinct types of cells in a tissue, we routinely grind up the entire tissue to isolate DNA, RNA, and proteins. Interestingly, the scientific community has been doing single cell analysis for a long time. Microscopy has allowed us tosee things like differences in morphology, expression levels, and cell health in single cells. Flow cytometry has been able to do the same, but at a higher throughput using fluorescent dyes and light scattering.