Surface Modification of Poly(dimethylsiloxane)Microchip by Using Cetyltrimethylammonium Bromide(CTAB)

摘要

In the analysis of many important biomolecules,a miniaturized microchip has shown a great potential in the areas of medicinal chemistry,biology,and environmental sciences.A microchip equipped with sample pretreatment,separation,and detection is especially called Lab-on-a-chip,and has extensively been utilized in the analysis of DNA,proteins,amino acids,and hormones.In the beginning,conventional materials such as quartz or glass were employed to fabricate the microchip.Although those chip materials revealed a good compatibility with traditional chemistry,fabrication process is labor-intensive and expensive.Furthermore,the bonding of upper and lower part of the chip is sometimes troublesome.Microchip produced by a plastic material such as poly(dimethylsiloxane)(PDMS)has shown a good material of choice since PDMS has good biocompati-bility,facile bonding ability,high transparency for UV and fluorescence detection,and is cost-effective for the produc-tion.