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首页> 外文期刊>International Journal of Pharmacy and Pharmaceutical Sciences >MOLECULAR IDENTIFICATION OF AEROMONAS SPP ISOLATED FROM PATIENTS WITH DIARRHEA AT SANTA MARIA-RS, BRAZIL
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MOLECULAR IDENTIFICATION OF AEROMONAS SPP ISOLATED FROM PATIENTS WITH DIARRHEA AT SANTA MARIA-RS, BRAZIL

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摘要

Objective: The aim of the study was to determine the frequency of Aeromonas spp. in stool samples of outpatients with gastroenteritis attended by clinical laboratories at Santa Maria-RS, Brazil. Methods: In order to evaluate this frequency, 767 clinical stool samples were processed by conventional methods as preconized, and suspected Aeromonas strains were submitted to molecular characterization by 16SrRNA PCR-RFLP method. Results: Aeromonas spp. were isolated from 14 (1.8) of stool cultures and identified as A. caviae (04), A. hydrophila (03), and A. veronii biovar sobria (01) by molecular method. Six strains presented atypical PCR-RFLP patterns, and therefore were identified as Aeromonas spp. Conclusion: Aeromonas is part of the bacteria associated with diarrhea in Santa Maria-RS, and results indicates that at least 3 Aeromonas species are involved with the disease. Keywords: Aeromonas , PCR-RFLP, Gastroenteritis

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