Bovine α_1-microglobulin/bikunin. Isolation and characterization of liver cDNA and urinary α_1-microglobulin

摘要

cDNA coding for a,-microglobulin, an immunoregulatory plasmaprotein, was isolated from bovine liver. The sequence of a total of 1258 nucleotides revealed an open reading frame of 352 amino acids. This included a,-microglobulin, 182 amino acids, and bikunin, the light chain of the plasmaprotein inter-a-inhibitor, 147 amino acids. The two proteins were connected by a basic tetrapeptide, R-A-R-R, whicli conforms to the consensus sequence recognized by endoproteolytic cleavage enzymes. The deduced amino acid sequence showed a high degree of identity with α_1-microglobulin and bikunin sequences from other species, and the α_1-microglobultn part displayed sequence motifs typical for members of the lipocalin protein superfamily. A single ai-microglobulin/bikunin mRNA with a size of around 1300 nt was found in bovine liver. The mature a,-microglobulin protein was isolated from bovine urine, and partly characterized. It was found to be a globular molecule with an apparent molecular weight of 23 300, containing one iV-linked and at least one O-linked oligosaccharide, one intra-chain disulfide bridge and an electrophoretic heterogeneity with a pI-value of 4.1-5.2.