Nanon effects on mammalian cell long-term cultures: a caveat for experimental hematologists dealing with in vitro long-term culture assays.

摘要

This letter aims to make cell culture investigators using human or animal long-term cell cultures aware of potential contamination by nanobacteria-like particles [1-3], recently proposed as nanons [4]. The real nature of these replicating particles has already been demonstrated in numerous studies. Raoult et al. [4], in particular, showed that these particles are self-propagating mineral-protein complexes, the major component of which is fetuin. Moreover, they demonstrated that nanon monolayer can be suppressed by ultraviolet/gamma irradiation, as well as by contact with either trypsin or ethylenediamine tetra-acetic acid [4].