Re: Franclim R. Ribeiro, Rui Henrique, Ana T. Martins, Carmen Jeronimo and Manuel R. Teixeira. Relative copy number gain of MYC in diagnostic needle biopsies is an independent prognostic factor for prostate cancer patients. Eur Urol 2007;52:116-25.

摘要

We read with particular interest the article by Ribeiro et al in the July 2007 issue of European Urology. Using a cohort of 60 patients with prostate cancer (archived paraffin-embedded biopsy sample blocks) and fluorescence in situ hybridization (FISH), they demonstrated that a gain of Sq was independently associated with poor disease-specific survival. They chose a dual-color probe flanking the MYC gene at Sq24.1 and controlled the ploidy of the sample with a centromeric probe for chromosome 18. Moreover, they affirm that instead of using a probe for chromosome 8, which is not readily available with a SpectrumAqua label, they used a centromeric probe for chromosome 18 and applied a MYC/CEP18 ratio that reflects the relative MYC copy number adjusted for the ploidy level of the sample.