Effects of cadmium and nitrogen on lipid composition of Chlorella vulgaris (Trebouxiophyceae, Chlorophyta)

摘要

Investigations were carried out to evaluate lipid class and fatty acid composition of Chlorella vulgaris in relation to several combinations of cadmium (2x108; 107M Cd) and nitrogen (2.9x106 to 1.1x103 M N) concentrations. Triacylglycerols (TAG), acetone mobile polar lipids (AMPL) and phospholipids (PL) were the major lipid classes of C. vulgaris under all the tested conditions. TAG was the lipid class accumulated in the highest amount in N-limited C. vulgaris. The AMPL lipid class was lowest at the lowest N and highest Cd treatment. High proportions of saturated fatty acids (SAFA) and monounsaturated fatty acids (MUFA) in the total lipid extracts were recorded under N limitation in the presence of Cd. 16:1(n-11) was an exception among the MUFA as its levels decreased at low N. Total polyunsaturated FA (PUFA), 3 PUFA levels, and PUFA:SAFA ratios increased with increasing N concentrations, but when exposed to Cd, their levels were significantly reduced. Unlike the other PUFA, 18:2(n-6) increased with increasing N limitation. Significant negative relationships between TAG, MUFA and SAFA with N were recorded. Cd had a positive correlation with TAG, while N was with AMPL, PL and PUFA. In conclusion, a combination of N limitation and Cd stress significantly altered the lipid composition of C. vulgaris, and N limitation had the most significant overall effect on lipid class and fatty acid composition of the microalga.