Ultrastructure and enzyme complement of proplastids from heterotrophically grown cells of the red alga Galdieria stilphuraria

摘要

The unicellular thermoacidophilic red alga Galdieria sulphur aria is known to grow autotrophically as well as heterotrophically on a wide variety of organic carbon sources. Strain 107.79 becomes nearly colourless when grown heterotrophically on lactose. Under autotrophic conditions, a dominant chloroplast occupies most of the cell volume, while very small, yellow to colourless plastid-like organelles were observed in heterotrophic cells. Their exact number could not be established with certainty. Toinvestigate the role of these algal proplastids, we developed a method to isolate intact organelles from heterotrophic cells via sucrose density-gradient centrifugation. Using electron microscopy, these organelles were shown to have a morphology similarto proplastids with a double membrane envelope, very few thylakoids and one to several dense bodies, apparently plastoglobuli. Investigation of the enzymatic capacities of isolated proplastids showed activity of triose phosphate isomerase and NAD-glyceraldehyde 3-phosphate dehydrogenase (GAPDH), but NADP-GAPDH, 3-phosphoglycerate kinase, fructose-1,6 P_2 aldolase, fructose-1,6 P_2 phosphatase, and malate dehydrogenase were not detected. These findings were supported by the pattern of plastid- and cytosol-specific isozymes of sugar phosphate metabolism and indicate that heterotrophically grown cells of G. sulphuraria 107.79 have not only lost their photosynthetic apparatus but also exhibit an extremely reduced enzyme complement in their dedifferentiatedplastid organelle.