IMMUNOHISTOCHEMICAL PROPERTIES OF CAUDAL MESENTERIC GANGLION AND ANTERIOR PELVICGANGLION NEURONS PROJECTING TO THE PORCINE TESTES IN ANIMALS SUBJECTED TO HEMICASTRATION, CASTRATION, AND TESTOSTERONE SUPPLEMENTATION

摘要

The present study was carried out on sexually mature boars. All the animals were injected with fast blue into right testis and then divided into four groups (Gl- control animals, G2 - hemicastrated, G3 - castrated, and G4 - castrated and injected withtestosterone). After 3 weeks, Gl pigs were transcardially perfused. In G2 pigs right testes, whereas in G3 and G4 animals both testes vvcre removed. G4 pigs were injected with testosterone. After 2 weeks, the pigs were transcardially perfused and then their caudal mesenteric ganglia (CaMG) and anterior pelvic ganglia (APG) were collected. The ganglia were cut into 12 mum-thick cryostat sections. Sections were stained using antisera against TH or DBH, VACHT or CHAT, NPY, VIP and GAL, and androgen receptor (AR). Immunohistochemical staining of CaMG-sections revealed that approximately 74% of FB-positive (FB~+) neurons contained immunoreactivity to TH or DBH, whereas 4% of FB~+ cells were VACHT-positive. Among FB~+/DbetaH~+ neurons, 72% contained NPY and2% stained for GAL. All FB~+ACHT~+ neurons were also VIP~+. 62% of FB~+ somata were NPY*, whereas 6% stained for VIP. In all experimental animals, numbers of FB~+ perikarya immunoreactive to TH (approx. 30%) and DBH (approx. 50%) were smaller than thosefound in Gl animals, whereas numbers of neurons displaying immunoreactivity to other substances studied were higher. The most significant increases regarded those expressing GAL (approx. 30%) and VIP (approx. 20%) whereas less distinct changes dealt with NPY~+ and VAChT~+ or ChAT~+ neurones. In APG, 60% of FB~+ neurons contained immunoreactivity to TH or DBH, whereas 12% of FB~+ cells were VACHT-positive. Among FB~+/DBH+ neurons, 55% contained NPY and 3% stained for GAL. All FB~+/VACHT~+ neurons were also VIP~+. 46% of FB~+ somata were NPY-IR, whereas 19% stained for VIP. In all experimental pigs, the iinnnmohistochemical properties of the APG FB~+ neurones were similar to those found in relation to CaMG-perikarya. Neurones m 0~+ both studied gangliawere surrounded by dense networks of VACHT-positive nerve fibres. The most apparent changes in the iiunvanohistochemical features of the FB~+ neurons evoked by bilateral castration were observed in G3 pigs; whereas changes found in 04 were very similar to those observed in G2 animals.