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Genetic Differentiation of Apricot (Prunus armeniaca L.) Cultivars with SSR Markers

机译:带有SSR标记的杏(Prunus armeniaca L.)品种的遗传分化

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A total of 44 apricot genotypes belonging to germ-plasm collection of Corvinus University (Budapest, Hungary), BOKU University (IOG, Vienna) and the Research Centre for Agriculture and Forestry Laimburg (Italy) were screened with 10 microsatellite primer pairs previously developed in apricot. All primers produced polymorphic amplification patterns, revealing 85 different alleles. The mean number of alleles per locus was 8,5. Out of them, 21 alleles occurred only once in the investigated samples. Theobserved heterozygosity for individual loci ranged from 0.9545 to 0.3182 with an average of 0.6604. A UPGMA dendrogram based on Nei's genetic distance divided the cultivars into two groups that reflect their geographic origin, one with genotypes originating from Europe and the other with genotypes from Central Asia. Implications of these data for the use of SSR markers as a tool for fingerprinting cultivars in breeder's right protection, but also in assisting breeding and selection programmes in apricotare discussed.
机译:共筛选了44种属于Corvinus大学(匈牙利布达佩斯),BOKU大学(IOG维也纳)和农林研究中心(意大利)种质资源的杏基因型,使用先前在10月开发的10个微卫星引物对进行了筛选。杏。所有引物均产生多态性扩增模式,揭示了85个不同的等位基因。每个基因座的等位基因平均数为8.5。在这些样本中,有21个等位基因在被调查的样本中仅出现一次。单个基因座的观察到的杂合度为0.9545至0.3182,平均为0.6604。基于Nei遗传距离的UPGMA树状图将品种分为反映其地理起源的两组,一组具有来自欧洲的基因型,另一组具有来自中亚的基因型。这些数据对于使用SSR标记作为育种者权利保护中的指纹识别品种的工具的含义,以及在协助杏子的育种和选择程序中的意义进行了讨论。

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