Coaxial PCL/PVA electrospun nanofibers: Osseointegration enhancer and controlled drug release device

摘要

The failure of prosthesis after total joint replacement is mainly due to dysfunctional osseointegration and implant infection. There is a critical need for orthopedic implants that promote rapid osseointegration and prevent bacterial colonization, particularly when placed in bone compromised by disease or physiology of the patients. The aim of this study was to fabricate a novel coaxial electrospun polycaprolactone (PCL)/polyvinyl alcohol (PVA) core-sheath nanofiber (NF) blended with both hydroxyapatite nanorods (HA) and type I collagen (Col) (PCL~(Col)/PVA~(HA)). Doxycycline (Doxy) and dexamethasone (Dex) were successfully incorporated into the PCL~(Col)/PVA~(HA) NFs for controlled release. The morphology, surface hydrophilicity and mechanical properties of the PCL/PVA NF mats were analyzed by scanning electron microscopy, water contact angle and atomic force microscopy. The PCL~(Col)/PVA~(HA) NFs are biocompatible and enhance the adhesion and proliferation of murine pre-osteoblastic MC3T3 cells. The release of Doxy and Dex from coaxial PCLCol/PVAHA NFs showed more controlled release compared with the blended NFs. Using an ex vivo porcine bone implantation model we found that the PCLCol/PVA HA NFs bind firmly on the titanium rod surface and the NFs coating remained intact on the surface of titanium rods after pullout. No disruption or delamination was observed after the pullout test. These findings indicate that PCL~(Col)/PVA~(HA) NFs encapsulating drugs have great potential in enhancing implant osseointegration and preventing implant infection.