Effect of light and temperature on in vitro germination of hybrid seed of pansy (Viola tricolor) and petunia (Petunia hybrida).

摘要

In an attempt to standardize the in vitro germination of hybrid pansy and petunia, seeds were inoculated in 1/2 MS media after disinfecting with HgCl2 (0.1%) for 8 minutes. The maximum percentage of germination (76.6%) in pansy was observed in dark condition at 18 C when half portion of the seed were placed into the 1/2 MS media where as in petunia maximum germination (86.6%) was observed in light condition at 24 C when seeds were placed on the surface of medium. Some germination in petunia was observed at each temperature except at 14, 16 and 16 C under light condition.