Man-Tailored Biomimetic Sensor of Molecularly Imprinted Materials for the Potentiometric Measurement of Rivastigmine in Tablets and Biological Fluids and Employing the Taguchi Optimization Methodology to Optimize the MIP-Based Membranes

摘要

This work proposes a novel biomimetic sensor for the potentiometric transduction of rivastigmine based on molecularly imprinted polymer (MIP). Using the Taguchi method, this study analyzed the optimum conditions for preparing the MIP-based membranes. The rank order of each controllable factor was also determined. MIP-based membranes exhibited a Nernstian response (30.7±1.1mV decade ~(-1)) in a concentration range from 1.0×10 ~(-5) to 1.0×10 ~(-2)molL ~(-1) with a LOD of 6.3×10 ~(-6)molL ~(-1). The sensor was successfully applied to the determination of rivastigmine concentrations in human serum, plasma, urine, rat brain and tablets.