Glucose-sensing electrode based on glucose oxidase-attached polyion complex membrane containing peroxidase and ferrocene

摘要

A glucose oxidase (GOD)-attached polyion complex membrane containing peroxidase (POD) and ferrocene was prepared by two steps on a glassy carbon electrode. First, a polyion complex membrane containing POD and ferrocene was prepared: a poly-L-lysine solution, a POD solution, a ferrocene dispersion and a poly(styrenesulfonate) solution were successively dropped on an electrode, and the electrode was allowed to dry. Then GOD was attached by the use of glutaraldehyde: GOD and glutaraldehyde solutions were dropped on the membrane. and the electrode was dried. Current response to glucose for the enzyme electrode was measured at a potential of -0.2 V (vs. Ag/AgCl). The 100 % response time was ca. 20 s. The current was proportional to the glucose concentration up to 0.1 mM. The lower detection limit was 1 muM (signal-to-noise ratio. S/N = 5). The enzyme electrode was used for the glucose measurements in beverages. The results obtained agreed well with those obtained by Boehringer F-kit method. The electrode would be useful as a glucose biosensor for beverages. [References: 34]