Genetic variability in the kappa-casein gene in Sahiwal, Cholistani and Red Sindhi cattle breeds.

摘要

Polymorphism of kappa-casein ( kappa-CN) gene in three Bos indicus cattle breeds was investigated using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Only genotypes AA and AB were observed, and no BB, AE, BE, EE, AC or BC genotypes were detected in the three cattle breeds. In the Sahiwal, Cholistani and Red Sindhi breeds, the frequencies of the allele A were 0.69, 0.90 and 0.86, and the frequencies of the allele B were 0.31, 0.10 and 0.14, respectively. The Sahiwal breed was found to have a higher frequency of the AB genotype as compared to the Cholistani and Red Sindhi breeds. The frequency of the kappa-CN alleles noted in the three cattle breeds was similar to those observed in other cattle breeds of B. indicus origin