Enhanced Mycelial Biomass Production of the Hairy Bracket Mushroom, Trametes hirsuta (Higher Basidiomycetes), by Optimizing Medium Component with Plackett-Burman Design and Response Surface Methodology

摘要

Statistical analyses based on experimental designs were applied to optimize the medium components for mycelial biomass production by Trametes hirsuta in shake flask cultivation. First, the effects of different carbon resources (glucose, sucrose, lactose, maltose, fructose, soluble starch and potato), nitrogen resources (yeast extract, peptone, (NH_4)_2SO_4, NH_4NO_3, NH_4C1, peanut powder, soybean powder) and mineral elements (CaCl_2, ZnSO_4.7H_2O, FeS_O4.7H_2O, MnSO_4 H_2O, CuSO_4 7H_2O) on mycelialbiomass production were investigated using a univariate design. Second, a Plackett-Burman design was applied to identify the significant variables that principally influenced the mycelial biomass production, and the path of steepest ascent was pursued to approach the regions of optimal value of the significant variables. Subsequently, these significant variables were optimized using the Box-Behnken design of response surface methodology. Ultimately, the optimized medium conditions were composed of sucrose 25.65 g L~（(-1)）, MgSO_4-7H_2O 1.24 g L~（(-1), and FeSO_4 7H_2O 3.36 g L~（(-1), and the yield of mycelial biomass reached 15.45 g L~（(-1), which represents an approximately 1.6-fold increase above the initial yield.