Plant regeneration through somatic embryogenesis of Asparagus officinalis

摘要

An efficient method for plant regeneration of Asparagus officinalis through somatic embryogenesis was developed. Callus was obtained from seedling segments on Murashige and Skoog (MS) medium supplemented with 1 mg/l naphthalene acetic acid (NAA) + 0.5 mg/l benzyleadenine (BA). The highest frequency of embryogenic callus and then conversion to embryos were observed with a medium containing 1 mg/l 2,4- dichlorophenoxy acetic acid (2,4-D) + 1 mg/l kinetin (kin). The highest percentage of embryo germination was obtained when the medium contained 0.5 mg/l 2,4-D + 0.5 mg/l kin was used. Among three concentrations of both adenine sulfate and casien hydrolysate added sperately to the germination medium; 80 mg/l of adenine sulfate obviously enhanced shootand root length. However, the highest percentage of embryo germination and highest number of roots were recorded when 2000 mg/l casien hydrolysate was added to the germination medium. The highest percentage of survival was obtained when plantlets in sizeof 5-10 cm shoot to 3-5 cm root were transplanted in peat moss and parlite (1:1).