Detection of RAPD polymorphism in anthurium cultivars with differing sensitivities to bacterial blight (Xanthomonas axonopodis pv. dieffenbachiae)

摘要

Xanthomonas axonopodis pv.dieffenbachiae (XAD) is the most important anthurium pathogen. One of the most environment friendly methods to control its spread is to use cultivars that are minimally sensitive to the pathogen. RAPD analysis of genomes frombulk segregant analysis of anthurium hybrids chosen to be the most tolerant and sensitive to XAD were performed to verify DNA polymorphism levels. RAPD analysis was performed using 110 primers with varying template DNA and primer concentrations. Six decamer primers produced 104 polymorphic bands, allowing RAPD profiles typical for one or more of the tolerant hybrids. The extent of polymorphism per primer varied from 61.90 to 88.23 per cent with a mean of 74.80 per cent. A dendrogram based on Squared Euclidian Distance grouped the anthurium in 3 major subgroups. Cloning the more interesting polymorphic DNA fragments in the future will verify the presence of specific genes related to XAD resistance mechanisms in anthurium.