Factors affecting pollen embryogenesis of rice anther culture

摘要

Anther culture techniques as a means of breeding are considered effective and time-saving methods for obtaining pure lines in rice and for other major crop improvement. Pollen embryogenesis is believed to provide new prospects for anther culture because of its stability and enhanced recovery of regenerants. We occasionally observed pollen embryogenesis during rice anther culture in many cultivars and F, plants in our laboratory. Growth regulators, water stress, and media composition affect the induction rate of pollen embryogenesis (Torrizo and Zapata l986). Abscisic acid (ABA) in the callus formation medium was most effective for pollen embryogenesis of rice; callus formation from the anthers plated did not increase significantly. However, embryo formations were markedly increased.