SDS-polyacrylamide gel electrophoresis of proteins of cauda epididymis fluid, vas deferens fluid and seminal plasma of buffalo bulls

摘要

Testes were obtained from 12 slaughtered buffalo bulls and cauda epididymal fluid and vas deferens washings were collected. In addition, 12 semen samples were collected from fertile buffaloes. Protein content of the samples was determined using SDS-PAGE. 16, 18 and 20 protein bands with molecular weights of 14-135 kDa were found in cauda epididymal fluid, vas deferens fluid and seminal plasma, respectively. Two proteins were exclusive to cauda epididymal fluid (29 and 24 kDa), 3 to vas deferens fluid (112, 37 and 25 kDa) and 2 to seminal plasma (120 and 115 kDa).