3D Scaffold with PCL Combined Biomedical Ceramic Materials for Bone Tissue Regeneration

摘要

Three-dimensional porous biodegradable polymer scaffolds have been widely used for tissue engineering of bone repair or regeneration. The primary function of scaffolds is to provide structure support for the cells adhesion and proliferation. This study selects the Poly-ε-caprolactone (PCL) as material, NaCl mixed with hydroxyapatite (HA) or nano-aluminum oxide (nAl_2O_3) for porous scaffold. This study uses the solvent casting/particulate leaching method to fabricate the porous scaffold. The authors discuss the compression mechanical properties, physical properties (porosity, moisture content, contact angle) of a pure PCL, PCL/mHA, PCLHA and PCLAl_2O_3 scaffolds. In vitro cell culture is used for osteoblast cell (MG63) and the microculture tetrazolium test (MTT) is undertaken in the scaffold. The scaffolds are implanted to the femur of rats and histological examination is attempted after 2 weeks. The experimental results indicate that HA and nAl_2O_3 can improve the hydrophilic property. In conclusion, the PCLHA scaffold exhibits splendid in vivo biocompatibility and osteogenesis.