Somatic Embryogenesis and Plant Regeneration from Nodal Explants in Psoralea corylifolia L.

摘要

The development of efficient in vitro regeneration systems are needed to facilitate the application of recombinant DNA technology to the improvement of crop germplasm. In the present study a simple, rapid and effective system to regenerate Psoralea corylifolia plants via direct somatic embryogenesis from nodal segments has been established. The embryogenic cells proliferated, formed somatic embryos, and were subsequently converted into normal plantlets under optimized culture conditions. The frequency of somatic embryogenesis was strongly influenced by the concentration of thidiazuron (TDZ) in the medium. The highest frequency (82%) of somatic embryogenesis was observed on Murashige and Skoog (MS) medium containing 16.0 muM TDZ. The somatic embryoswhen transferred to plant growth regulator-free MS basal medium, developed further to heart shaped, torpedo and cotyledonary stages within 2 weeks. Conversion of somatic embryos into plantlets was achieved by isolating somatic embryos with distinct cotyledons and transferring them onto half-strength MS medium containing 1.0 uM gibberellic acid (GA_3). Subsequently, the regenerated plantlets were successfully established in ex vitro condition with 90% survival. This is the first report on in vitro regeneration via direct somatic embryogenesis of P. corylifolia.