The presence of multiple rat DSP-PP transcripts

摘要

Phosphoproteins or phosphophoryns (PPs) are the most abundant (> 50%) non-collagenous proteins (NCPs) in dentin. PPs bind to calcium and hydroxyapatite and are believed to play a crucial role in dentin mineralization. Dentin sialoprotein (DSP), a highly glycosylated protein, comprised 5-8% of NCPs in dentin. The coding sequences for these two major NCPs are known to be contiguously located (i.e. DSP-PP) at the cDNA and genomic DNA levels in both rat and mouse. Previous studies have demonstrated the presence of multiple DSP-PP transcripts in the total RNA of adult rat incisors. To further understand the nature of these multiple transcripts, we performed reverse transcription-PCR and obtained a PP cDNA variant which encoded a 171 amino acid peptide (PP_(171)) that shares many of the same characteristics as that of the published rat PP_(240) sequence [Ritchie, H. H. and Wang, L.-H., J. Biol. Chem. 271 (1996) 21695-21698]. Due to its reduced size, as compared to P_(240), this cDNA encodes a phosphorylated protein with a reduced negative charge that may differentially affect mineralization processes. We provide evidence that there are multiple DSP-PP transcripts with various sizes of PP sequences in rat.