Utility of spermidine in PCR amplification of stool samples.

摘要

When nucleic acid is amplified from clinical samples by PCR, it is necessary to suppress the action of any inhibitory substances in the amplification reaction. Inhibitory substances of PCR are present in clinical samples such as stool, and in some cases inhibitors of PCR cannot be removed (Wilson 1997; Verweij et al, 2000).