Biochemical effect of three molluscicide baits against the two land snails, Monacha cantiana and Eobania vermiculata (Gastropoda: Helicidae).

摘要

The present study was investigated the biochemical effect of three molluscicides in the tissues of the two land snails, Monacha cantiana (Montagu) and Eobania vermiculata (Muller). The field recommended rate of three commercial molluscicide baits namely: Gastrotox, mlotov and mesurol were evaluated. The activities of five vital enzymes, total protein (TP) and total lipid (TL) were laboratory tested. The five vital enzymes were acetylcholine-esterase (AchE), alanine amino transaminase (ALT), aspartate amino transaminase (AST), lactate dehydrogenase (LDH) and mixed function oxidase (MFO). Results showed that all tested molluscicides lead to increase the activity of AST, LDH and MFO in the tested land snails, E. vermiculata and M. cantiana, except mesurol treatment showed a decrease in MFO when applied against the two land snails. All of the tested molluscicides had inhibitory effect on the activity of AchE in both tested land snails. The results also revealed that all tested molluscicides increased the activity of ALT in the tissue homogenate of the land snail, M. cantiana. Obviously, all of the tested molluscicides decreased the activity of ALT in the land snail, E. vermiculata. On the other hand, the levels of total protein and total lipid were increased after treatment with all tested molluscicides when applied against E. vermiculata. Moreover, the levels of total protein and total lipid decreased after treatment in the tissue homogenate of the land snail, M. cantiana. In general, the three molluscicides were significantly affected on the activities of enzymes, total lipid and total protein compared with control treatment when applied against the tested snails.