PROTECTIVE EFFECT OF A POTENTIAL POLYHERBAL DRUG ON MAMMARY TISSUE DAMAGE CAUSED BY INTRAMAMMARY ADMINISTRATION OF ANTIBIOTIC BY PRODUCING ANTIOXIDANT ACTION

摘要

The study was aimed to evaluate the effect of intramammary administration of ceftriaxone (antibiotic) on milk enzyme activites to assess mammary gland tissue damage and oxidative stress and to observe the effect of a poly herbal drug (Fibrosin~R) on milk enzyme activites by one hour prior oral dosing to single intramammary administration of ceftriaxone. A total of twelve healthy lactating goats were divided into two groups (Gr-I and Gr-II) each containing six goats. A single dose of ceftriaxone dissolving in 5 ml of distilled water was divided into 2 equal portions and administered to each quarter of goats of Gr-I intracisternally at 50 mg/kg body weight A half bolus of Fibrosin8 was orally administered one hour prior to intramammary administrationof ceftriaxone at 50 mg/kg body weight to goats of Gr-II. Milk alkaline phospatase activity was increased gradually until 96 hour for the antibiotic treated group indicating mammary gland tissue damage. However, the enzyme activity returned to its normallevel in Fibrosin~R treated Gr-II goats at 96 hour postdosing of Fibrosin~R. Only intramammary antibiotic administration lowered milk GSH level at 6 hour, but thereafter steadily increased reduced glutathione (GSH) level until 96 hour. But the polyherbal treatment maintained a normal range of milk reduced glutathione. Milk Catalase activity gradually increased until 48 hour and decreased afterward until 96 hour postdosing for group L Whereas the enzyme activity increased until 24 hour, afterward decreased at 48 hour and again increased from 72 hour to 96 hour with the polyherbal treatment signifying antioxidant activity of Fibrosin~R.