COMPARISON OF DETECTION THRESHOLD OF DIFFERENT PASTEURELLA,MULTOCIDA SPECIFIC PCRs

摘要

The present study was undertaken to determine the detection threshold of five different Pasteurella multocida PCRs using a standard vaccine strain P52 as DNA template. The purified genomic DNA was diluted ten-fold serially and then subject to polymerase chain reaction. Among the primers assessed, IPFWD or IPREV primers showed the highest sensitivity, detecting purified DNA at 5 fg concentration (-2 bacterial cells). KMT1T7 & KMT1SP6, KTSP61 & KTT72 and PSL-(forward & reverse) primers could detect P52DNA at the levels of 500, 50 and 50 pg respectively. PM23F1 & PM23R2 primers were the least sensitive and could detect P52 DNA at 5 ng concentration only.