Wound dressing based on chitosan/hyaluronanonwoven fabrics: Preparation, characterization and medical applications

摘要

Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)onwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CFA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrateonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/FIA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronanonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample. (C) 2016 Elsevier B.V. All rights reserved.