Antifouling potential of a marine strain, Pseudomonas aeruginosa 1242, isolated from brass microfouling in Vietnam.

摘要

Due to increased concern about the adverse effects of biocides on the environment, the creation of environmentally friendly coatings is now of considerable interest. This work is yet another attempt to use the enormous potential of marine microorganisms to protect surfaces from fouling. A strain of Pseudomonas aeruginosa 1242 was selected for this study after screening of different microorganisms for ability to inhibit the growth of microorganisms; this strain appeared to be highly active. Using the enzymatic approach, we have chosen the medium and cultivation conditions for the strain 1242, which ensured the maximum production of hydrolytic exoenzymes. The cells and metabolite complex of the strain were then embedded into an epoxy-resin-based commercial anticorrosion coating. It was shown in laboratory experiments that the bacterial cells introduced into the epoxy coating remain viable for a sufficiently long time. The results of trials of the experimental coatings in seawaters of different climatic zones demonstrated that the cells of this strain and the complex of enzymes and secondary metabolites that they produced could prevent adhesion of micro- and macrofoulers.