Morphological, Cytological and Biochemical Characterization of wheat Aegilops Longissima Derivatives BC_1F_6 and BC_2F_4 with High Grain Micronutrient

摘要

Micronutrient deficiency (Iron and Zinc) is the major problem worldwide mostly in the developing countries with high dependence on staple foods. Biofortification of staple cereal and tuber crops has been considered and taken up as the most effective,feasible and economic approach for alleviating micronutrient deficiency. The present study was the initiative towards biofortification of wheat where the previous work of wide hybridization between HD2687 and Aegilops longissima accession 3506 and subsequent backcrossing with Triticitm aestivum cultivar WL711 has been continued. In this study the alien chromosome introgression, chromosomal stability of BC_1F_6and BC_2F_4 wheatse. longissima derivatives has been investigated for their potential as germplasm for future breeding and biofortification program through morphological, cytological and biochemical analysis. We found that the selected derivatives showed stable 42, 44 and 46 chromosomes for most of the plants where single plant reported for each 41, 43 and 45 chromosomes with 19-22 bivalents and few trivalent. The GISH analysis of derivative 79-1-4-8-1-2-2 revealed introgression of two univalent S1 chromosomes. Finally four derivatives were selected from BC_1F_6 (79-1-4-8-10-2-2, 79-1-4-8-10-2-5,79-2-4-4-1-1-3 and 79-2-4-4-1-1-5) and three from BC_2F_4 (HD2687/L3506//WL711-3//AVL711-1-2-7-1, HD2687/L3506/AVL711-3///WL711-1-2-7-3, HD2687/L3506//WL711-3//AVL711-1-2-7-5) as stable biofortified lines for future breeding to alleviate hidden hunger.