A batch decolorization and kinetic study of Reactive Black 5 by a bacterial strain Enterobacter sp. GY-1.

摘要

An Enterobacter strain (GY-1) with high activity of decolorization of Reactive Black 5 (RB 5) was isolated from textile wastewater treating sludge. The kinetic characteristics of dye decolorization by the strain GY-1 were determined quantitatively using the diazo dye, RB 5. Effects of different operation parameters (inoculum size, pH, temperature and salinity) and various electron donors on decolorization of the azo dye by GY-1 were systematically investigated to reveal the primary factors that determine the performance of the azo dye decolorization. The decolorization of RB 5 was attributed to extracellular enzymes. A kinetic model was established giving the dependence of decolorization rate on cell mass concentration (first order). Decolorization rate increased with increasing temperature from 20 to 35 degrees C, which can be predicted by Arrhenius equation with the activation energy (E_a) of 8.50 kcal mol-1 and the frequency factor (A₀) of 6.28x107 mg l g MLSS-1 h-1. Michaelis-Menten kinetics and Eadie-Hofstee plot were used to determine V_max, 1.05 mg l-1 h-1 and K_m, 24.06 mg l-1.Digital Object Identifier http://dx.doi.org/10.1016/j.ibiod.2011.05.003