The function of an installed photosynthetic formaldehyde-assimilation pathway containing dihydroxyacetone synthase and dihydroxyacetone kinase enhanced formaldehyde removal from solution in transgenic geranium leaves

摘要

In a previous study, we created transgenic geranium leaves installed with a photosynthetic formaldehyde (HCHO)-assimilation pathway containing dihydroxyacetone synthase (DAS) and dihydroxyacetone kinase (DAM). In the current study, DAS and DAM expression was confirmed in the leaves of three selected transgenic lines via RT-PCR analysis. C-13 NMR analysis showed that the actions of the DAS/DAK pathway completely inhibited the role of citrate-produced pathway during HCHO metabolism in transgenic geranium leaves treated with 2 mM (HCHO)-C-13 for 4 h. Further analysis indicated that HCHO uptake from this solution was always higher in the leaves of the three transgenic lines than in WT (untransformed) leaves during a 48-h treatment period. This result suggests that the role of the installed DAS/DAK pathway enhanced HCHO uptake from solution in transgenic geranium leaves. Moreover, the relationship between HCHO uptake and treatment time approached a linear function during the first 40-h treatment period due to the function of the installed pathway. Based on the (HCHO)-C-13 metabolic profile and the HCHO uptake curves, (HCHO)-C-13 metabolic pathways in transgenic leaves treated with 2 mM (HCHO)-C-13 were postulated and the contribution of the DAS/DAK pathway to HCHO uptake by transgenic leaves was quantitatively evaluated. (C) 2015 Elsevier Ltd. All rights reserved.