Expression in lymphocyte and fibroblast culture of the fragile X chromosome: A new technical approach

摘要

By studying those conditions most favourable for the expression of the fragile site fra(X)(q28) in lymphocytes and in fibroblasts, we have been able to develop a reliable and standardised technique. The technique which gave the best results in lymphocytes was culture in TC 199 for 96 h with addition of methotrexate 10 mg/l for the final 24 h. These same two factors, prolongation of the culture time in TC 199 and addition of methotrexate, gave satisfactory results in fibroblasts.