The release properties of silver ions from Ag-nHA/TiO2/PA66 antimicrobial composite scaffolds

摘要

Implant-associated bacterial infection can jeopardize the clinical success of implants and result in loss of supporting bone. The purpose of this study was to develop a novel porous scaffold with long-term antibacterial activity for bone repair or regeneration. Porous nano-hydroxyapatite/titania/polyamide66 scaffolds containing different amounts of silver ions (Ag-nHA/TiO2/PA66) were prepared by a phase inversion technique. The release of silver ions from the porous scaffolds in simulated body fluid (SBF) and in the F12 cell culture medium was evaluated via atomic absorption spectrometry. The results showed that the release of Ag+ was time and concentration dependent, increasing with the immersion time and the silver content in the scaffolds. On the other hand, the release property of Ag+ was also influenced by the immersion medium. The cumulative Ag+ release in the F12 medium with time increase parabolically, different from the linear increase or the zero-order release kinetics in the SBF medium. Compared to the slight fluctuation of the Ag+ release rate in SBF during the whole immersion period, the initial fast release rate and the later sustained release rate of Ag+ in the F12 medium could be more helpful for preventing implant-associated infection. Since the Ag-nHA/TiO2 particles were embedded in the PA66 matrix, the long-term-sustained release should be related both to the relaxation of PA macromolecular chains due to the penetration of water and to the slow release of the substituted Ag+ ions in the HA lattice. The sustained Ag+ release with time indicates that the composite scaffold is suitable for a long-term antimicrobial application during the scaffold-assisted bone repair or regeneration.