Replication and innate host response of influenza A virus in lung microvascular endothelial cells: new insights into systemic infection and pathogenesis

摘要

Though influenza A virus replication kinetics and host responses have been previously studied in umbilical vein endothelial cell or transformed endothelial cell lines, the tropism of influenza A virus including H5N1 and pandemic H1Nlpdm for primary human lung microvascular endothelial cell has not been well defined. In this study we employed primary human lung microvascular endothelial cells, which are more physiologically relevant for understanding pathogenesis of influenza in the lung as to obtain a better understanding of the links of endothelial cell infection to systematic virus dissemination and multiple organ involvement in severe human influenza.