A single space polymerase chain reaction for combined gene detection andepidemiological typing of Listeria monocytogenes

摘要

A variant of the random amplification of polymorphic DNA (RAPD) technique, referred to as the 'combined gene detection and epidemiological typing' (COGEDET) method was employed in the development of a one-step PCR assay for simultaneous identification and typing of Listeria monocytogenes. An arbitrary (RAPD) primer was used in combination with a species specific gene primer set. Four gene primers, HLYA, PRFA, ISP and FL2A, and six RAPD primers were screened for their discriminatory abilities.