Detection of Potato DNA from Potato Tubers and Processed Food Products by Means of PCR

摘要

A new method for specific and sensitive detection of potato DMA (Solanum tuberosum) in processed potato food products has been developed using poly-merase chain reaction (PCR). A nested PCR system amplifying a 146 bp fragment of the patatin gene of Solanum tuberosum was evolved. Quality and amount of DNA in potato products were determined by gel-electrophoresis and absorption at 260 nm. Several potato cultivars authorised in Switzerland and highly processed food products like instant potato soup, instant mashed potatoes, potato crisps and crackers and pommes frites were investigated. For all samples the 146 bp fragment could be amplified with the new nested PCR system.