Fractionation is an essential step in the identification of proteins and their subsequent structural characterization. This information, in turn, is vital to understanding protein participation and function in normal and pathological cellular processes. The vast range in protein abundance (at least 6 orders of magnitude in dynamic range), differences in solubility, and relative amenability to separation by established methods (which are often time consuming and manually intensive) have created the need for new approaches to fractionation that can overcome these limitations. Specifically, problems that should be addressed include.
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