AbstractThree sugars were tried as carbon sources for the synthesis of total lipids, triglycerides and sterols inFusarium oxysporum.Glucose at 3 level gave the maximum. Polar lipids in the mycelium varied from 4.2 to 9.7. An increase in glucose concentration led to increased production of saturated fatty acids. Nine salts containing either the ammonium or nitrate ions were used as a nitrogen source. Potassium nitrate was best for lipid synthesis and ammonium bicarbonate yielded polar‐rich lipids. The content of free fatty acid was relatively higher on ammonium salts. Except for the minor changes in the fatty acid make‐up, no definite differences could be observed. The optimum pH was found to be 5.5. At this pH, triglycerides and free sterols were in considerably higher concentration. The proportion of linoleic acid remained more or less constant between the pH range of 3.5 and 6.5 while that of linolenic acid showed the maximum value at pH of 4.5. C:N ratios of 30 and 300 were found to be the optimum among narrow and wide ran
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