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Effect of different cultural conditions on the chemical composition of lipids offusarium oxysporum

机译:不同培养条件对尖孢脂质化学成分的影响

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AbstractThree sugars were tried as carbon sources for the synthesis of total lipids, triglycerides and sterols inFusarium oxysporum.Glucose at 3 level gave the maximum. Polar lipids in the mycelium varied from 4.2 to 9.7. An increase in glucose concentration led to increased production of saturated fatty acids. Nine salts containing either the ammonium or nitrate ions were used as a nitrogen source. Potassium nitrate was best for lipid synthesis and ammonium bicarbonate yielded polar‐rich lipids. The content of free fatty acid was relatively higher on ammonium salts. Except for the minor changes in the fatty acid make‐up, no definite differences could be observed. The optimum pH was found to be 5.5. At this pH, triglycerides and free sterols were in considerably higher concentration. The proportion of linoleic acid remained more or less constant between the pH range of 3.5 and 6.5 while that of linolenic acid showed the maximum value at pH of 4.5. C:N ratios of 30 and 300 were found to be the optimum among narrow and wide ran
机译:摘要尝试将3种糖作为碳源合成尖孢镰刀菌中总脂质、甘油三酯和甾醇。葡萄糖在3%水平给出了最大值。菌丝体中的极性脂质从4.2%到9.7%不等。葡萄糖浓度的增加导致饱和脂肪酸的产生增加。使用含有铵离子或硝酸根离子的九种盐作为氮源。硝酸钾最适合脂质合成,碳酸氢铵产生富含极性的脂质。铵盐上游离脂肪酸含量相对较高。除了脂肪酸组成的微小变化外,没有观察到明显的差异。发现最佳pH值为5.5。在这个pH值下,甘油三酯和游离甾醇的浓度要高得多。在pH值为3.5至6.5的pH值范围内,亚油酸的比例基本保持不变,而亚麻酸的比例在pH值为4.5时最大值。发现30和300的C:N比在窄跑和宽跑中是最佳的

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