Inhibitory effects of chlorogenic acid and its related compounds on the invasion of hepatoma cells in culture

摘要

Actions of chlorogenic acid, a major component of coffee, and its constituents, caffeic and quinic acids, on the proliferation and invasion of AH109A, a rat ascites hepatoma cell line, were investigated using in vitro assay systems. All three components suppressed the AH109A invasion at concentrations of 5-40 mu M without altering the cell proliferation. At the concentration of 10 mu M, chlorogenic, caffeic and quinic acids significantly (P < 0.05) suppressed the invasion by 68, 36 and 31, respectively, implying that the suppressive effect of chlorogenic acid on the AH109A invasion might result from the additive effects of its constituents, caffeic and quinic acids. At the concentration of 10 mu M, cinnamic acid and p-coumaric acid (4-hydroxycinnamic acid) exerted no or little influence on the invasion, whereas caffeic acid (3,4-dihydroxycinnamic acid) significantly (P < 0.05) suppressed it, suggesting the possible involvement of the 3,4-dihydroxy group of caffeic acid in the suppression. Chlorogenic acid was thus demonstrated to be one of the chemical entities in coffee suppressing the hepatoma invasion in vitro, and both of its constituents, caffeic and quinic acids, to be responsible for the anti-invasive activity. These results suggest the existence of nutritionally and pharmacologically important substances in coffee which control tumor cell invasion. References: 20