PHENOTYPIC CHARACTERIZATION OF A HUMAN SYNOVIAL SARCOMA CELL LINE, SW982, AND ITS RESPONSE TO DEXAMETHASONE

摘要

SW982 cells are characterized by expression of inflammatory cytokine and matrix metalloproteinase (MMP) genes and by their response to dexamethasone at different cell densities. They express genes encoding interleukin (IL)-1β; IL-6; transforming growth factor–β; intercellular adhesion molecule–1; cycloxygenase (COX)-2; and MMPs, including MMP-1, MMP-2, MMP-13, and MT1-MMP; tissue inhibitor of metalloproteinase–2; and a disintegrin and metalloproteinase with thrombospondin motifs–4. Expression of all the genes examined was induced with 2 ng/ml IL-1β at low cell density. The cells, however, failed to express tumor necrosis factor–α, COX-1, and MMP-9, regardless of the presence of IL-1β. Dexamethasone significantly reduced IL-1β, IL-6, COX-2, and MMP-1β expression at high cell density. The results suggest that SW982 cells are a useful tool for studying the expression of inflammatory cytokine or MMP genes.