Micropropagation of Valeriana wallichii DC. (Indian Valerian) through nodes

摘要

In vitro propagation method was developed for obtaining large number of plantlets of Valeriana wallichii DC. (Valerianaceae), an indigenous high value medicinal and aromatic plant species of Indian Himalayan region, using nodes of in vitro grown seedlings. High frequency shoot proliferation was induced in explains cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine (BA), kinetin (Kn), adenine sulphate (AS) and thidiazuron (TDZ). Amongst all the tested cytokinins, 3.0 mu M BA was found to be the most effective and an average. of 12.0 shoots per nodal explant were formed after 15 d of culture. Higher concentration of TDZ (3.0 mu M) was found least effective among all the cytokinins used for shoot induction. Rooting (100 %) was observed in PGR-free Murashige and Skoog medium after 12 d of incubation of microshoots. Well rooted plantlets (30-d-old) were successfully transplanted and established in mixture of soil and sand (3:1) under partially shade conditions.