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首页> 外文期刊>Avian Diseases >Mixed Infections of Marek's Disease and Reticuloendotheliosis Viruses inLayer Flocks in Argentina
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Mixed Infections of Marek's Disease and Reticuloendotheliosis Viruses inLayer Flocks in Argentina

机译:马雷克病和网状内皮病病毒在阿根廷鸡群中的混合感染

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The presence of reticuloendotheliosis virus (REV) was examined in flocks affected with Marek's disease (MD). Sera were positive to REV antibodies by agar gel precipitation. However, these findings were not conclusive since fowlpox vaccines can have REV fragments or the whole genome inserted. Frozen sections from tumors were positive for MD virus (MDV) but negative for REV. Chicken embryo fibroblast (CEF) and chicken kidney cell (CKC) culture inoculated with buffy coat cells or blood from the affectedbirds were examined. Positive cells were shown for REV and MDV by fluorescent antibodies tests in CEF and CKC, respectively, indicating the presence of REV in Argentinean layer flocks. This is the first report of REV in Argentina and also in South America.
机译:在受马立克氏病(MD)影响的鸡群中检查了网状内皮病病毒(REV)的存在。通过琼脂凝胶沉淀,血清对REV抗体呈阳性。但是,由于禽痘疫苗可以插入REV片段或插入整个基因组,因此这些发现尚无定论。肿瘤的冷冻切片对MD病毒(MDV)呈阳性,但对REV呈阴性。检查了接种了血沉棕黄层细胞或患病鸟血液的鸡胚成纤维细胞(CEF)和鸡肾细胞(CKC)培养物。分别在CEF和CKC中通过荧光抗体测试显示了阳性细胞的REV和MDV,表明阿根廷鸡群中存在REV。这是REV在阿根廷和南美的第一份报告。

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